Construct: shRNA TRCN0000328634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008008.4-1318s21c1
DNA Barcode:
GATGTTAGTAACGTGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgf7 (14178)
Gene Description:
fibroblast growth factor 7
Transcript:
RefSeq NM_008008.4 (CURRENT)
Match location:
Position 1318 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTTAGTAACGTGATATTT
Hairpin Sequence:
5'-CCGG-GATGTTAGTAACGTGATATTT-CTCGAG-AAATATCACGTTACTAACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTTAGTAACGTGATATTTCTCGAGAAATATCACGTTACTAACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTTAGTAACGTGATATTTCTCGAGAAATATCACGTTACTAACATC-3'

Other clones with same target sequence:

(none)