Transcript: Mouse NM_008008.4

Mus musculus fibroblast growth factor 7 (Fgf7), mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Mus musculus (mouse)
Gene:
Fgf7 (14178)
Length:
2672
CDS:
464..1048

Additional Resources:

NCBI RefSeq record:
NM_008008.4
NBCI Gene record:
Fgf7 (14178)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008008.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000067677 GAAATGTTCGTTGCCTTAAAT pLKO.1 947 CDS 100% 15.000 21.000 N Fgf7 n/a
2 TRCN0000328634 GATGTTAGTAACGTGATATTT pLKO_005 1318 3UTR 100% 15.000 21.000 N Fgf7 n/a
3 TRCN0000328710 ACACCTATGCATCAGCTAAAT pLKO_005 909 CDS 100% 13.200 18.480 N Fgf7 n/a
4 TRCN0000328636 CCTAAGTGTACATGGTATTTG pLKO_005 1044 CDS 100% 13.200 18.480 N Fgf7 n/a
5 TRCN0000067676 GTCTGTCTAGTGGGCACTATA pLKO.1 527 CDS 100% 13.200 10.560 N Fgf7 n/a
6 TRCN0000328711 TCTGTCTAGTGGGCACTATAT pLKO_005 528 CDS 100% 13.200 10.560 N Fgf7 n/a
7 TRCN0000067675 CACCAGAAGTTATGACTACAT pLKO.1 619 CDS 100% 4.950 3.960 N Fgf7 n/a
8 TRCN0000328635 GAGCGACACACCAGAAGTTAT pLKO_005 611 CDS 100% 13.200 9.240 N Fgf7 n/a
9 TRCN0000067673 GCCTGTACTGACTAATATGAA pLKO.1 1652 3UTR 100% 5.625 3.938 N Fgf7 n/a
10 TRCN0000067674 GTTGGAATTGTGGCAATCAAA pLKO.1 776 CDS 100% 5.625 3.938 N Fgf7 n/a
11 TRCN0000159283 GCAAAGAAAGAATGCAATGAA pLKO.1 848 CDS 100% 5.625 3.375 N FGF7P6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008008.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10821 pDONR223 100% 44.5% 44.3% None (many diffs) n/a
2 ccsbBroad304_10821 pLX_304 0% 44.5% 44.3% V5 (many diffs) n/a
3 TRCN0000467676 TCACACTCCGTCTTATAAACCGCG pLX_317 100% 44.5% 44.3% V5 (many diffs) n/a
4 TRCN0000488797 AATTAACGATGCTTCAATCTAAGG pLX_317 96.5% 44.5% 44.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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