Construct: shRNA TRCN0000328711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008008.4-528s21c1
DNA Barcode:
TCTGTCTAGTGGGCACTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgf7 (14178)
Gene Description:
fibroblast growth factor 7
Transcript:
RefSeq NM_008008.4 (CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGTCTAGTGGGCACTATAT
Hairpin Sequence:
5'-CCGG-TCTGTCTAGTGGGCACTATAT-CTCGAG-ATATAGTGCCCACTAGACAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGTCTAGTGGGCACTATATCTCGAGATATAGTGCCCACTAGACAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGTCTAGTGGGCACTATATCTCGAGATATAGTGCCCACTAGACAGA-3'

Other clones with same target sequence:

(none)