Construct: shRNA TRCN0000328773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099628.1-2832s21c1
DNA Barcode:
CGAAGAACAGTACCGAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atad2b (320817)
Gene Description:
ATPase family, AAA domain containing 2B
Transcript:
RefSeq NM_001099628.1 (CURRENT)
Match location:
Position 2832 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGAACAGTACCGAGTATT
Hairpin Sequence:
5'-CCGG-CGAAGAACAGTACCGAGTATT-CTCGAG-AATACTCGGTACTGTTCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGAACAGTACCGAGTATTCTCGAGAATACTCGGTACTGTTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGAACAGTACCGAGTATTCTCGAGAATACTCGGTACTGTTCTTCG-3'

Other clones with same target sequence:

(none)