Construct: shRNA TRCN0000328862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099628.1-1561s21c1
DNA Barcode:
GTGGATTGAGCCATCATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atad2b (320817)
Gene Description:
ATPase family, AAA domain containing 2B
Transcript:
RefSeq NM_001099628.1 (CURRENT)
Match location:
Position 1561 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGATTGAGCCATCATATTC
Hairpin Sequence:
5'-CCGG-GTGGATTGAGCCATCATATTC-CTCGAG-GAATATGATGGCTCAATCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGATTGAGCCATCATATTCCTCGAGGAATATGATGGCTCAATCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGATTGAGCCATCATATTCCTCGAGGAATATGATGGCTCAATCCAC-3'

Other clones with same target sequence:

  1. TRCN0000336621