Construct: shRNA TRCN0000329438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026529.4-232s21c1
DNA Barcode:
CCAAAGATACAGAAACTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2700062C07Rik (68046)
Gene Description:
RIKEN cDNA 2700062C07 gene
Transcript:
RefSeq NM_026529.4 (CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGATACAGAAACTTCTT
Hairpin Sequence:
5'-CCGG-CCAAAGATACAGAAACTTCTT-CTCGAG-AAGAAGTTTCTGTATCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGATACAGAAACTTCTTCTCGAGAAGAAGTTTCTGTATCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGATACAGAAACTTCTTCTCGAGAAGAAGTTTCTGTATCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000176564