Construct: shRNA TRCN0000329497

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028339.1-1257s21c1
DNA Barcode:
GCCCATTATTTCAAGACATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmx1 (72736)
Gene Description:
thioredoxin-related transmembrane protein 1
Transcript:
RefSeq NM_028339.1 (CURRENT)
Match location:
Position 1257 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329497)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTATTTCAAGACATAT
Hairpin Sequence:
5'-CCGG-GCCCATTATTTCAAGACATAT-CTCGAG-ATATGTCTTGAAATAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTATTTCAAGACATATCTCGAGATATGTCTTGAAATAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTATTTCAAGACATATCTCGAGATATGTCTTGAAATAATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000114326