Construct: shRNA TRCN0000329555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181732.4-800s21c1
DNA Barcode:
CTATTCCATTAAGACGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aida (108909)
Gene Description:
axin interactor, dorsalization associated
Transcript:
RefSeq NM_181732.4 (CURRENT)
Match location:
Position 800 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTCCATTAAGACGTATTT
Hairpin Sequence:
5'-CCGG-CTATTCCATTAAGACGTATTT-CTCGAG-AAATACGTCTTAATGGAATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTCCATTAAGACGTATTTCTCGAGAAATACGTCTTAATGGAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTCCATTAAGACGTATTTCTCGAGAAATACGTCTTAATGGAATAG-3'

Other clones with same target sequence:

(none)