Construct: shRNA TRCN0000329563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007888.3-241s21c1
DNA Barcode:
TAGGCGAGACGAAGGTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dvl2 (13543)
Gene Description:
dishevelled segment polarity protein 2
Transcript:
RefSeq NM_007888.3 (CURRENT)
Match location:
Position 241 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGCGAGACGAAGGTGATTT
Hairpin Sequence:
5'-CCGG-TAGGCGAGACGAAGGTGATTT-CTCGAG-AAATCACCTTCGTCTCGCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGCGAGACGAAGGTGATTTCTCGAGAAATCACCTTCGTCTCGCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGCGAGACGAAGGTGATTTCTCGAGAAATCACCTTCGTCTCGCCTA-3'

Other clones with same target sequence:

(none)