Construct: shRNA TRCN0000329629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007888.3-1293s21c1
DNA Barcode:
TACTTCACCCTCCCTCGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dvl2 (13543)
Gene Description:
dishevelled segment polarity protein 2
Transcript:
RefSeq NM_007888.3 (CURRENT)
Match location:
Position 1293 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000329629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTTCACCCTCCCTCGAAAT
Hairpin Sequence:
5'-CCGG-TACTTCACCCTCCCTCGAAAT-CTCGAG-ATTTCGAGGGAGGGTGAAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTTCACCCTCCCTCGAAATCTCGAGATTTCGAGGGAGGGTGAAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTTCACCCTCCCTCGAAATCTCGAGATTTCGAGGGAGGGTGAAGTA-3'

Other clones with same target sequence:

(none)