Construct: shRNA TRCN0000331487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009805.4-779s21c1
DNA Barcode:
CCTCACCTGGTTTCTGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cflar (12633)
Gene Description:
CASP8 and FADD-like apoptosis regulator
Transcript:
RefSeq NM_009805.4 (CURRENT)
Match location:
Position 779 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACCTGGTTTCTGATTAT
Hairpin Sequence:
5'-CCGG-CCTCACCTGGTTTCTGATTAT-CTCGAG-ATAATCAGAAACCAGGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACCTGGTTTCTGATTATCTCGAGATAATCAGAAACCAGGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACCTGGTTTCTGATTATCTCGAGATAATCAGAAACCAGGTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000031008