Construct: shRNA TRCN0000331586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198023.2-826s21c1
DNA Barcode:
GTCTCCATCAAACGACAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rcor1 (217864)
Gene Description:
REST corepressor 1
Transcript:
RefSeq NM_198023.2 (CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCCATCAAACGACAGATT
Hairpin Sequence:
5'-CCGG-GTCTCCATCAAACGACAGATT-CTCGAG-AATCTGTCGTTTGATGGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCCATCAAACGACAGATTCTCGAGAATCTGTCGTTTGATGGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCCATCAAACGACAGATTCTCGAGAATCTGTCGTTTGATGGAGAC-3'

Other clones with same target sequence:

  1. TRCN0000071372