Construct: shRNA TRCN0000331948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003908.1-4643s21c1
DNA Barcode:
GCGAACATCAATAGATGCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cltc (67300)
Gene Description:
clathrin, heavy polypeptide (Hc)
Transcript:
RefSeq NM_001003908.1 (CURRENT)
Match location:
Position 4643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000331948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAACATCAATAGATGCTTA
Hairpin Sequence:
5'-CCGG-GCGAACATCAATAGATGCTTA-CTCGAG-TAAGCATCTATTGATGTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAACATCAATAGATGCTTACTCGAGTAAGCATCTATTGATGTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAACATCAATAGATGCTTACTCGAGTAAGCATCTATTGATGTTCGC-3'

Other clones with same target sequence:

  1. TRCN0000113161