Construct: shRNA TRCN0000332016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133987.2-1436s21c1
DNA Barcode:
CGTGTACTTCACTGCTACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a8 (102857)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Transcript:
RefSeq NM_133987.2 (CURRENT)
Match location:
Position 1436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTACTTCACTGCTACATT
Hairpin Sequence:
5'-CCGG-CGTGTACTTCACTGCTACATT-CTCGAG-AATGTAGCAGTGAAGTACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTACTTCACTGCTACATTCTCGAGAATGTAGCAGTGAAGTACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTACTTCACTGCTACATTCTCGAGAATGTAGCAGTGAAGTACACG-3'

Other clones with same target sequence:

  1. TRCN0000079859