Construct: shRNA TRCN0000332019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033314.3-2710s21c1
DNA Barcode:
CCACCACTTTGGGAAGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco2a1 (24059)
Gene Description:
solute carrier organic anion transporter family, member 2a1
Transcript:
RefSeq NM_033314.3 (CURRENT)
Match location:
Position 2710 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCACTTTGGGAAGTATAA
Hairpin Sequence:
5'-CCGG-CCACCACTTTGGGAAGTATAA-CTCGAG-TTATACTTCCCAAAGTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCACTTTGGGAAGTATAACTCGAGTTATACTTCCCAAAGTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCACTTTGGGAAGTATAACTCGAGTTATACTTCCCAAAGTGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000079868