Construct: shRNA TRCN0000332078

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133987.2-1012s21c1
DNA Barcode:
GCAGCATCAATGTCTGGAACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a8 (102857)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Transcript:
RefSeq NM_133987.2 (CURRENT)
Match location:
Position 1012 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332078)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATCAATGTCTGGAACA
Hairpin Sequence:
5'-CCGG-GCAGCATCAATGTCTGGAACA-CTCGAG-TGTTCCAGACATTGATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATCAATGTCTGGAACACTCGAGTGTTCCAGACATTGATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATCAATGTCTGGAACACTCGAGTGTTCCAGACATTGATGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000079861