Construct: shRNA TRCN0000332197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175294.3-304s21c1
DNA Barcode:
GTTGTGGATTACTCTCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nucks1 (98415)
Gene Description:
nuclear casein kinase and cyclin-dependent kinase substrate 1
Transcript:
RefSeq NM_175294.3 (CURRENT)
Match location:
Position 304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGTGGATTACTCTCAGTTT
Hairpin Sequence:
5'-CCGG-GTTGTGGATTACTCTCAGTTT-CTCGAG-AAACTGAGAGTAATCCACAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGTGGATTACTCTCAGTTTCTCGAGAAACTGAGAGTAATCCACAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGTGGATTACTCTCAGTTTCTCGAGAAACTGAGAGTAATCCACAAC-3'

Other clones with same target sequence:

  1. TRCN0000099346