Construct: shRNA TRCN0000332517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011293.2-384s21c1
DNA Barcode:
GCCATCAAGGACAAGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polr2j (20022)
Gene Description:
polymerase (RNA) II (DNA directed) polypeptide J
Transcript:
RefSeq NM_011293.2 (CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCAAGGACAAGCAAGAA
Hairpin Sequence:
5'-CCGG-GCCATCAAGGACAAGCAAGAA-CTCGAG-TTCTTGCTTGTCCTTGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCAAGGACAAGCAAGAACTCGAGTTCTTGCTTGTCCTTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCAAGGACAAGCAAGAACTCGAGTTCTTGCTTGTCCTTGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000111371