Construct: shRNA TRCN0000332649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022433.2-400s21c1
DNA Barcode:
GCCATCTTTGAACTTGGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sirt3 (64384)
Gene Description:
sirtuin 3
Transcript:
RefSeq NM_022433.2 (CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCTTTGAACTTGGCTTT
Hairpin Sequence:
5'-CCGG-GCCATCTTTGAACTTGGCTTT-CTCGAG-AAAGCCAAGTTCAAAGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCTTTGAACTTGGCTTTCTCGAGAAAGCCAAGTTCAAAGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCTTTGAACTTGGCTTTCTCGAGAAAGCCAAGTTCAAAGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000039329