Transcript: Mouse NM_022433.2

Mus musculus sirtuin 3 (Sirt3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-14
Taxon:
Mus musculus (mouse)
Gene:
Sirt3 (64384)
Length:
1484
CDS:
295..1068

Additional Resources:

NCBI RefSeq record:
NM_022433.2
NBCI Gene record:
Sirt3 (64384)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_022433.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039332 GCCCAATGTCACTCACTACTT pLKO.1 483 CDS 100% 4.950 3.960 N Sirt3 n/a
2 TRCN0000332647 GCCCAATGTCACTCACTACTT pLKO_005 483 CDS 100% 4.950 3.960 N Sirt3 n/a
3 TRCN0000039330 GCGGCTCTATACACAGAACAT pLKO.1 537 CDS 100% 4.950 3.960 N Sirt3 n/a
4 TRCN0000306513 AGACAGCTCCAACACGTTTAC pLKO_005 1139 3UTR 100% 10.800 7.560 N Sirt3 n/a
5 TRCN0000039331 CCTACTCCATATGGCTGACTT pLKO.1 774 CDS 100% 0.000 0.000 N Sirt3 n/a
6 TRCN0000039333 CACAAGAACTGCTGGATCTTA pLKO.1 1007 CDS 100% 5.625 3.375 N Sirt3 n/a
7 TRCN0000332648 CACAAGAACTGCTGGATCTTA pLKO_005 1007 CDS 100% 5.625 3.375 N Sirt3 n/a
8 TRCN0000039329 GCCATCTTTGAACTTGGCTTT pLKO.1 400 CDS 100% 4.050 2.430 N Sirt3 n/a
9 TRCN0000332649 GCCATCTTTGAACTTGGCTTT pLKO_005 400 CDS 100% 4.050 2.430 N Sirt3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022433.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02761 pDONR223 98.6% 54% 55.1% None (many diffs) n/a
2 ccsbBroad304_02761 pLX_304 0% 54% 55.1% V5 (many diffs) n/a
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