Construct: shRNA TRCN0000332792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001135559.1-4654s21c1
DNA Barcode:
GCCAGTATAAAGACTCTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sos2 (20663)
Gene Description:
son of sevenless homolog 2 (Drosophila)
Transcript:
RefSeq NM_001135559.1 (CURRENT)
Match location:
Position 4654 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTATAAAGACTCTGTAT
Hairpin Sequence:
5'-CCGG-GCCAGTATAAAGACTCTGTAT-CTCGAG-ATACAGAGTCTTTATACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTATAAAGACTCTGTATCTCGAGATACAGAGTCTTTATACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTATAAAGACTCTGTATCTCGAGATACAGAGTCTTTATACTGGC-3'

Other clones with same target sequence:

  1. TRCN0000076953