Construct: shRNA TRCN0000332800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012056.2-495s21c1
DNA Barcode:
CCTACGGAAGTGAAGGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fkbp9 (27055)
Gene Description:
FK506 binding protein 9
Transcript:
RefSeq NM_012056.2 (CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGGAAGTGAAGGAGTTT
Hairpin Sequence:
5'-CCGG-CCTACGGAAGTGAAGGAGTTT-CTCGAG-AAACTCCTTCACTTCCGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGGAAGTGAAGGAGTTTCTCGAGAAACTCCTTCACTTCCGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGGAAGTGAAGGAGTTTCTCGAGAAACTCCTTCACTTCCGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000111852