Construct: shRNA TRCN0000332972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002755.3-979s21c1
DNA Barcode:
AGTTAGCATTGCTGTAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K1 (5604)
Gene Description:
mitogen-activated protein kinase kinase 1
Transcript:
RefSeq NM_002755.3 (CURRENT)
Match location:
Position 979 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000332972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTAGCATTGCTGTAATAAA
Hairpin Sequence:
5'-CCGG-AGTTAGCATTGCTGTAATAAA-CTCGAG-TTTATTACAGCAATGCTAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTAGCATTGCTGTAATAAACTCGAGTTTATTACAGCAATGCTAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTAGCATTGCTGTAATAAACTCGAGTTTATTACAGCAATGCTAACT-3'

Other clones with same target sequence:

  1. TRCN0000219684