Construct: shRNA TRCN0000333643

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017829.5-282s21c1
DNA Barcode:
AGGTGGATGCAGACCAAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDHD5 (27440)
Gene Description:
haloacid dehalogenase like hydrolase domain containing 5
Transcript:
RefSeq NM_017829.5 (CURRENT)
Match location:
Position 282 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333643)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTGGATGCAGACCAAGTTA
Hairpin Sequence:
5'-CCGG-AGGTGGATGCAGACCAAGTTA-CTCGAG-TAACTTGGTCTGCATCCACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTGGATGCAGACCAAGTTACTCGAGTAACTTGGTCTGCATCCACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTGGATGCAGACCAAGTTACTCGAGTAACTTGGTCTGCATCCACCT-3'

Other clones with same target sequence:

  1. TRCN0000050204