Construct: shRNA TRCN0000333992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008652.2-1005s21c1
DNA Barcode:
CCAGAATCTCTCAAGCGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mybl2 (17865)
Gene Description:
myeloblastosis oncogene-like 2
Transcript:
RefSeq NM_008652.2 (CURRENT)
Match location:
Position 1005 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000333992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAATCTCTCAAGCGTGAA
Hairpin Sequence:
5'-CCGG-CCAGAATCTCTCAAGCGTGAA-CTCGAG-TTCACGCTTGAGAGATTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAATCTCTCAAGCGTGAACTCGAGTTCACGCTTGAGAGATTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAATCTCTCAAGCGTGAACTCGAGTTCACGCTTGAGAGATTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000042508