Construct: shRNA TRCN0000334007

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009323.2-1729s21c1
DNA Barcode:
CGCGTCTCAAAGCACTTTACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbx15 (21384)
Gene Description:
T-box 15
Transcript:
RefSeq NM_009323.2 (CURRENT)
Match location:
Position 1729 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334007)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGTCTCAAAGCACTTTACT
Hairpin Sequence:
5'-CCGG-CGCGTCTCAAAGCACTTTACT-CTCGAG-AGTAAAGTGCTTTGAGACGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTCTCAAAGCACTTTACTCTCGAGAGTAAAGTGCTTTGAGACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTCTCAAAGCACTTTACTCTCGAGAGTAAAGTGCTTTGAGACGCG-3'

Other clones with same target sequence:

  1. TRCN0000084360