Construct: shRNA TRCN0000334014
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025980.2-458s21c1
- DNA Barcode:
- GAACATGACTAACTGCGAATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nrarp (67122)
- Gene Description:
- Notch-regulated ankyrin repeat protein
- Transcript:
- RefSeq NM_025980.2 (CURRENT)
- Match location:
- Position 458 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000334014)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAACATGACTAACTGCGAATT
- Hairpin Sequence:
-
5'-CCGG-GAACATGACTAACTGCGAATT-CTCGAG-AATTCGCAGTTAGTCATGTTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAACATGACTAACTGCGAATTCTCGAGAATTCGCAGTTAGTCATGTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAACATGACTAACTGCGAATTCTCGAGAATTCGCAGTTAGTCATGTTC-3'
Other clones with same target sequence:
-
TRCN0000085114
