Construct: shRNA TRCN0000334188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023644.4-1837s21c1
DNA Barcode:
GCAGATTGACAACAAGTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mccc1 (72039)
Gene Description:
methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
Transcript:
RefSeq NM_023644.4 (CURRENT)
Match location:
Position 1837 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTGACAACAAGTCCTT
Hairpin Sequence:
5'-CCGG-GCAGATTGACAACAAGTCCTT-CTCGAG-AAGGACTTGTTGTCAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTGACAACAAGTCCTTCTCGAGAAGGACTTGTTGTCAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTGACAACAAGTCCTTCTCGAGAAGGACTTGTTGTCAATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000112509