Construct: shRNA TRCN0000334198
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_033581.3-4371s21c1
- DNA Barcode:
- CGCTGGTGTAGAATAGCCAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pcdhgc3 (93706)
- Gene Description:
- protocadherin gamma subfamily C, 3
- Transcript:
- RefSeq NM_033581.3 (CURRENT)
- Match location:
- Position 4371 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000334198)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGCTGGTGTAGAATAGCCAAT
- Hairpin Sequence:
-
5'-CCGG-CGCTGGTGTAGAATAGCCAAT-CTCGAG-ATTGGCTATTCTACACCAGCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGCTGGTGTAGAATAGCCAATCTCGAGATTGGCTATTCTACACCAGCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGCTGGTGTAGAATAGCCAATCTCGAGATTGGCTATTCTACACCAGCG-3'
Other clones with same target sequence:
-
TRCN0000094374
