Construct: shRNA TRCN0000334222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207209.2-2848s21c1
DNA Barcode:
GCATATTCTACTATCCATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec24b (99683)
Gene Description:
Sec24 related gene family, member B (S. cerevisiae)
Transcript:
RefSeq NM_207209.2 (CURRENT)
Match location:
Position 2848 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATATTCTACTATCCATCTT
Hairpin Sequence:
5'-CCGG-GCATATTCTACTATCCATCTT-CTCGAG-AAGATGGATAGTAGAATATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATATTCTACTATCCATCTTCTCGAGAAGATGGATAGTAGAATATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATATTCTACTATCCATCTTCTCGAGAAGATGGATAGTAGAATATGC-3'

Other clones with same target sequence:

  1. TRCN0000100591