Construct: shRNA TRCN0000334259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026321.4-624s21c1
DNA Barcode:
GATGAGAAGAAGAAACCGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam174a (67698)
Gene Description:
family with sequence similarity 174, member A
Transcript:
RefSeq NM_026321.4 (CURRENT)
Match location:
Position 624 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334259)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGAAGAAGAAACCGCAA
Hairpin Sequence:
5'-CCGG-GATGAGAAGAAGAAACCGCAA-CTCGAG-TTGCGGTTTCTTCTTCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGAAGAAGAAACCGCAACTCGAGTTGCGGTTTCTTCTTCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGAAGAAGAAACCGCAACTCGAGTTGCGGTTTCTTCTTCTCATC-3'

Other clones with same target sequence:

  1. TRCN0000127061