Construct: shRNA TRCN0000334292

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008968.3-1528s21c1
DNA Barcode:
GACACAGAGGTTCCTGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptgis (19223)
Gene Description:
prostaglandin I2 (prostacyclin) synthase
Transcript:
RefSeq NM_008968.3 (CURRENT)
Match location:
Position 1528 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334292)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACACAGAGGTTCCTGAGTTT
Hairpin Sequence:
5'-CCGG-GACACAGAGGTTCCTGAGTTT-CTCGAG-AAACTCAGGAACCTCTGTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACACAGAGGTTCCTGAGTTTCTCGAGAAACTCAGGAACCTCTGTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACACAGAGGTTCCTGAGTTTCTCGAGAAACTCAGGAACCTCTGTGTC-3'

Other clones with same target sequence:

  1. TRCN0000067862