Transcript: Mouse NM_008968.3

Mus musculus prostaglandin I2 (prostacyclin) synthase (Ptgis), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Ptgis (19223)
Length:
1751
CDS:
118..1623

Additional Resources:

NCBI RefSeq record:
NM_008968.3
NBCI Gene record:
Ptgis (19223)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008968.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000067858 CTGGAGAGTTATCTGAGACAT pLKO.1 886 CDS 100% 4.950 3.960 N Ptgis n/a
2 TRCN0000334224 CTGGAGAGTTATCTGAGACAT pLKO_005 886 CDS 100% 4.950 3.960 N Ptgis n/a
3 TRCN0000067859 GCCTGAGGTGTTTAAATACAA pLKO.1 1320 CDS 100% 5.625 3.938 N Ptgis n/a
4 TRCN0000334225 GCCTGAGGTGTTTAAATACAA pLKO_005 1320 CDS 100% 5.625 3.938 N Ptgis n/a
5 TRCN0000067861 GCGCTCACAGAAGCCATGTAT pLKO.1 523 CDS 100% 5.625 3.938 N Ptgis n/a
6 TRCN0000334291 GCGCTCACAGAAGCCATGTAT pLKO_005 523 CDS 100% 5.625 3.938 N Ptgis n/a
7 TRCN0000067860 CAGCTTCCTTACCAGGATGAA pLKO.1 273 CDS 100% 4.950 3.465 N Ptgis n/a
8 TRCN0000067862 GACACAGAGGTTCCTGAGTTT pLKO.1 1528 CDS 100% 4.950 3.465 N Ptgis n/a
9 TRCN0000334292 GACACAGAGGTTCCTGAGTTT pLKO_005 1528 CDS 100% 4.950 3.465 N Ptgis n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008968.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01334 pDONR223 100% 83.8% 85.8% None (many diffs) n/a
2 ccsbBroad304_01334 pLX_304 0% 83.8% 85.8% V5 (many diffs) n/a
3 TRCN0000481284 CGATCTCTAGACCCTGATGTGCAA pLX_317 25% 83.8% 85.8% V5 (many diffs) n/a
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