Construct: shRNA TRCN0000334862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025478.3-829s21c1
DNA Barcode:
CCACCCGAAGTTCAAGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Isoc1 (66307)
Gene Description:
isochorismatase domain containing 1
Transcript:
RefSeq NM_025478.3 (CURRENT)
Match location:
Position 829 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCGAAGTTCAAGGAAAT
Hairpin Sequence:
5'-CCGG-CCACCCGAAGTTCAAGGAAAT-CTCGAG-ATTTCCTTGAACTTCGGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCGAAGTTCAAGGAAATCTCGAGATTTCCTTGAACTTCGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCGAAGTTCAAGGAAATCTCGAGATTTCCTTGAACTTCGGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000098977