Construct: shRNA TRCN0000334917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010344.4-1527s21c1
DNA Barcode:
GCTGTTCATAAGTATGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gsr (14782)
Gene Description:
glutathione reductase
Transcript:
RefSeq NM_010344.4 (CURRENT)
Match location:
Position 1527 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000334917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTCATAAGTATGGGAAA
Hairpin Sequence:
5'-CCGG-GCTGTTCATAAGTATGGGAAA-CTCGAG-TTTCCCATACTTATGAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTCATAAGTATGGGAAACTCGAGTTTCCCATACTTATGAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTCATAAGTATGGGAAACTCGAGTTTCCCATACTTATGAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000042339