Construct: shRNA TRCN0000335029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021356.2-1554s21c1
DNA Barcode:
CCAGAGCAAACACGGAATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gab1 (14388)
Gene Description:
growth factor receptor bound protein 2-associated protein 1
Transcript:
RefSeq NM_021356.2 (CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGCAAACACGGAATGAA
Hairpin Sequence:
5'-CCGG-CCAGAGCAAACACGGAATGAA-CTCGAG-TTCATTCCGTGTTTGCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGCAAACACGGAATGAACTCGAGTTCATTCCGTGTTTGCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGCAAACACGGAATGAACTCGAGTTCATTCCGTGTTTGCTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000100112