Construct: shRNA TRCN0000100112
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_021356.2-1554s1c1
- DNA Barcode:
- CCAGAGCAAACACGGAATGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gab1 (14388)
- Gene Description:
- growth factor receptor bound protein 2-associated protein 1
- Transcript:
- RefSeq NM_021356.2 (CURRENT)
- Match location:
- Position 1554 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000100112)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGAGCAAACACGGAATGAA
- Hairpin Sequence:
-
5'-CCGG-CCAGAGCAAACACGGAATGAA-CTCGAG-TTCATTCCGTGTTTGCTCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGAGCAAACACGGAATGAACTCGAGTTCATTCCGTGTTTGCTCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGAGCAAACACGGAATGAACTCGAGTTCATTCCGTGTTTGCTCTGG-3'
Other clones with same target sequence:
-
TRCN0000335029
