Construct: shRNA TRCN0000335398

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007592.3-804s21c1
DNA Barcode:
CCCTTTAACTATATCCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Car8 (12319)
Gene Description:
carbonic anhydrase 8
Transcript:
RefSeq NM_007592.3 (CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335398)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTAACTATATCCCAGAT
Hairpin Sequence:
5'-CCGG-CCCTTTAACTATATCCCAGAT-CTCGAG-ATCTGGGATATAGTTAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTAACTATATCCCAGATCTCGAGATCTGGGATATAGTTAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTAACTATATCCCAGATCTCGAGATCTGGGATATAGTTAAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000114515