Transcript: Mouse NM_007592.3

Mus musculus carbonic anhydrase 8 (Car8), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Car8 (12319)
Length:
5722
CDS:
88..963

Additional Resources:

NCBI RefSeq record:
NM_007592.3
NBCI Gene record:
Car8 (12319)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007592.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000114513 CCAGTCACCTATCAACCTAAA pLKO.1 234 CDS 100% 10.800 15.120 N Car8 n/a
2 TRCN0000335396 CCAGTCACCTATCAACCTAAA pLKO_005 234 CDS 100% 10.800 15.120 N Car8 n/a
3 TRCN0000114512 CGAAGGAGTTACCTGGATATT pLKO.1 774 CDS 100% 13.200 10.560 N Car8 n/a
4 TRCN0000335397 CGAAGGAGTTACCTGGATATT pLKO_005 774 CDS 100% 13.200 10.560 N Car8 n/a
5 TRCN0000114511 CCCATCTGCATCAGTGAAGAA pLKO.1 984 3UTR 100% 4.950 3.465 N Car8 n/a
6 TRCN0000114515 CCCTTTAACTATATCCCAGAT pLKO.1 804 CDS 100% 4.050 2.835 N Car8 n/a
7 TRCN0000335398 CCCTTTAACTATATCCCAGAT pLKO_005 804 CDS 100% 4.050 2.835 N Car8 n/a
8 TRCN0000114514 GCTTAGTGTTTCCTGATGCTA pLKO.1 203 CDS 100% 3.000 2.100 N Car8 n/a
9 TRCN0000335464 GCTTAGTGTTTCCTGATGCTA pLKO_005 203 CDS 100% 3.000 2.100 N Car8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007592.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15371 pDONR223 0% 89.5% 98.6% None (many diffs) n/a
2 ccsbBroad304_15371 pLX_304 0% 89.5% 98.6% V5 (many diffs) n/a
3 TRCN0000470274 AATAGACTGCACTAGCTTCGTGAT pLX_317 36.8% 89.5% 98.6% V5 (many diffs) n/a
4 ccsbBroadEn_00200 pDONR223 100% 89.4% 98.6% None (many diffs) n/a
5 ccsbBroad304_00200 pLX_304 0% 89.4% 98.6% V5 (many diffs) n/a
6 TRCN0000474299 CCCGGTTCTTGCTCCAAGCGACGC pLX_317 32% 89.4% 98.6% V5 (many diffs) n/a
7 ccsbBroadEn_10705 pDONR223 100% 45% 47.9% None (many diffs) n/a
8 ccsbBroad304_10705 pLX_304 0% 45% 47.9% V5 (many diffs) n/a
9 TRCN0000467276 ACCTTCAATTATTCTCGGTAAAAT pLX_317 96% 45% 47.9% V5 (many diffs) n/a
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