Construct: shRNA TRCN0000335664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027334.3-273s21c1
DNA Barcode:
CGGAGCCAACTTCAAGTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl7a1 (70152)
Gene Description:
methyltransferase like 7A1
Transcript:
RefSeq NM_027334.3 (CURRENT)
Match location:
Position 273 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGCCAACTTCAAGTTCTA
Hairpin Sequence:
5'-CCGG-CGGAGCCAACTTCAAGTTCTA-CTCGAG-TAGAACTTGAAGTTGGCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGCCAACTTCAAGTTCTACTCGAGTAGAACTTGAAGTTGGCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGCCAACTTCAAGTTCTACTCGAGTAGAACTTGAAGTTGGCTCCG-3'

Other clones with same target sequence:

  1. TRCN0000097563