Construct: shRNA TRCN0000335735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024438.4-513s21c1
DNA Barcode:
TCTATACTGGATGTGCCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp19 (68082)
Gene Description:
dual specificity phosphatase 19
Transcript:
RefSeq NM_024438.4 (CURRENT)
Match location:
Position 513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000335735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTATACTGGATGTGCCTGAA
Hairpin Sequence:
5'-CCGG-TCTATACTGGATGTGCCTGAA-CTCGAG-TTCAGGCACATCCAGTATAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTATACTGGATGTGCCTGAACTCGAGTTCAGGCACATCCAGTATAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTATACTGGATGTGCCTGAACTCGAGTTCAGGCACATCCAGTATAGA-3'

Other clones with same target sequence:

  1. TRCN0000081191