Construct: shRNA TRCN0000336022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178625.3-1781s21c1
DNA Barcode:
TGCGCACCTCTTCTGACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem209 (72649)
Gene Description:
transmembrane protein 209
Transcript:
RefSeq NM_178625.3 (NON-CURRENT)
Match location:
Position 1781 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGCACCTCTTCTGACATAA
Hairpin Sequence:
5'-CCGG-TGCGCACCTCTTCTGACATAA-CTCGAG-TTATGTCAGAAGAGGTGCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGCACCTCTTCTGACATAACTCGAGTTATGTCAGAAGAGGTGCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGCACCTCTTCTGACATAACTCGAGTTATGTCAGAAGAGGTGCGCA-3'

Other clones with same target sequence:

(none)