Construct: shRNA TRCN0000336205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007924.2-1745s21c1
DNA Barcode:
TGAGAAGCGACGCTGCGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ell (13716)
Gene Description:
elongation factor RNA polymerase II
Transcript:
RefSeq NM_007924.2 (CURRENT)
Match location:
Position 1745 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGAAGCGACGCTGCGAATA
Hairpin Sequence:
5'-CCGG-TGAGAAGCGACGCTGCGAATA-CTCGAG-TATTCGCAGCGTCGCTTCTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGAAGCGACGCTGCGAATACTCGAGTATTCGCAGCGTCGCTTCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGAAGCGACGCTGCGAATACTCGAGTATTCGCAGCGTCGCTTCTCA-3'

Other clones with same target sequence:

(none)