Construct: shRNA TRCN0000336224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138953.2-2390s21c1
DNA Barcode:
CTTTGTCATTAGCGATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ell2 (192657)
Gene Description:
elongation factor RNA polymerase II 2
Transcript:
RefSeq NM_138953.2 (CURRENT)
Match location:
Position 2390 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGTCATTAGCGATGATTT
Hairpin Sequence:
5'-CCGG-CTTTGTCATTAGCGATGATTT-CTCGAG-AAATCATCGCTAATGACAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGTCATTAGCGATGATTTCTCGAGAAATCATCGCTAATGACAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGTCATTAGCGATGATTTCTCGAGAAATCATCGCTAATGACAAAG-3'

Other clones with same target sequence:

(none)