Construct: shRNA TRCN0000336407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177460.4-1078s21c1
DNA Barcode:
TGAAGTACCTGCTGGTGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parp16 (214424)
Gene Description:
poly (ADP-ribose) polymerase family, member 16
Transcript:
RefSeq NM_177460.4 (CURRENT)
Match location:
Position 1078 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGTACCTGCTGGTGTATT
Hairpin Sequence:
5'-CCGG-TGAAGTACCTGCTGGTGTATT-CTCGAG-AATACACCAGCAGGTACTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGTACCTGCTGGTGTATTCTCGAGAATACACCAGCAGGTACTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGTACCTGCTGGTGTATTCTCGAGAATACACCAGCAGGTACTTCA-3'

Other clones with same target sequence:

(none)