Construct: shRNA TRCN0000336524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008695.2-2120s21c1
DNA Barcode:
CGAATTTCACAGCCCACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nid2 (18074)
Gene Description:
nidogen 2
Transcript:
RefSeq NM_008695.2 (CURRENT)
Match location:
Position 2120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000336524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATTTCACAGCCCACATTA
Hairpin Sequence:
5'-CCGG-CGAATTTCACAGCCCACATTA-CTCGAG-TAATGTGGGCTGTGAAATTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATTTCACAGCCCACATTACTCGAGTAATGTGGGCTGTGAAATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATTTCACAGCCCACATTACTCGAGTAATGTGGGCTGTGAAATTCG-3'

Other clones with same target sequence:

  1. TRCN0000114783