Construct: shRNA TRCN0000337294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001143980.1-1701s21c1
DNA Barcode:
TCATCCGTGCAGCTGCTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC154 (645811)
Gene Description:
coiled-coil domain containing 154
Transcript:
RefSeq NM_001143980.1 (CURRENT)
Match location:
Position 1701 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCCGTGCAGCTGCTAAAG
Hairpin Sequence:
5'-CCGG-TCATCCGTGCAGCTGCTAAAG-CTCGAG-CTTTAGCAGCTGCACGGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCCGTGCAGCTGCTAAAGCTCGAGCTTTAGCAGCTGCACGGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCCGTGCAGCTGCTAAAGCTCGAGCTTTAGCAGCTGCACGGATGA-3'

Other clones with same target sequence:

(none)