Construct: shRNA TRCN0000337579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009981.4-1488s21c1
DNA Barcode:
TGTAATCTTGACAGGGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcyt1a (13026)
Gene Description:
phosphate cytidylyltransferase 1, choline, alpha isoform
Transcript:
RefSeq NM_009981.4 (CURRENT)
Match location:
Position 1488 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000337579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAATCTTGACAGGGAAATT
Hairpin Sequence:
5'-CCGG-TGTAATCTTGACAGGGAAATT-CTCGAG-AATTTCCCTGTCAAGATTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAATCTTGACAGGGAAATTCTCGAGAATTTCCCTGTCAAGATTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAATCTTGACAGGGAAATTCTCGAGAATTTCCCTGTCAAGATTACA-3'

Other clones with same target sequence:

(none)