Construct: shRNA TRCN0000338045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033037.3-356s21c1
DNA Barcode:
ATTCGATCAATACAGGTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdo1 (12583)
Gene Description:
cysteine dioxygenase 1, cytosolic
Transcript:
RefSeq NM_033037.3 (CURRENT)
Match location:
Position 356 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCGATCAATACAGGTATAC
Hairpin Sequence:
5'-CCGG-ATTCGATCAATACAGGTATAC-CTCGAG-GTATACCTGTATTGATCGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCGATCAATACAGGTATACCTCGAGGTATACCTGTATTGATCGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCGATCAATACAGGTATACCTCGAGGTATACCTGTATTGATCGAAT-3'

Other clones with same target sequence:

(none)