Construct: shRNA TRCN0000338134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054089.4-968s21c1
DNA Barcode:
ACACAGAAGTAGACCAGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgs1 (116940)
Gene Description:
trimethylguanosine synthase 1
Transcript:
RefSeq NM_054089.4 (CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACAGAAGTAGACCAGAATG
Hairpin Sequence:
5'-CCGG-ACACAGAAGTAGACCAGAATG-CTCGAG-CATTCTGGTCTACTTCTGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACAGAAGTAGACCAGAATGCTCGAGCATTCTGGTCTACTTCTGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACAGAAGTAGACCAGAATGCTCGAGCATTCTGGTCTACTTCTGTGT-3'

Other clones with same target sequence:

(none)